fiberless multicolor neural optoelectrode for in vivo circuit analysis - pcb assembly services

In the deep brain structure of intact animals, maximizing the potential of light development methods requires optical manipulation of neurons at high spatial and temporal resolutions, while recording the electrical data of these neurons.
Here we show the first fiber.
With a smaller photoelectric pole with a monolithic integrated optical waveguide mixer, it can provide multi-color light at the common waveguide port to achieve multi-color modulation of the same neuron population in the body.
We demonstrate that the successful implementation of effective coupling between devices is performed on one side
Laser diode for emission injection (ILD)
And a optical waveguide mixer via gradient media-index (GRIN)lens.
The use of the GRIN lens implements a variety of design features, including high optical coupling and thermal isolation between the ILDs and the waveguide.
We verified the packaged device in the whole brain of the anesthetic mouse
The expression channel is purple and red-
2 and Archaerhodopsin in cone cells in hippocampus a1 region, achieving high quality recording, activation and silence of exactly the same neurons in a given local region. This fully-
The comprehensive approach demonstrates the spatial accuracy and scalability required to record the same or different groups of neurons independently activated and silenced in dense brain regions, and therefore, greatly improved the ability of the currently available set of light development tools.
Preparation of nerve probes ()
It started with silicon. on-Insulator (SOI)
Wafer with 22 μm thick device layer. An LPCVD (low-
Pressure Chemical vapor deposition)
In order to play the role of stress compensation and electrical insulation layer, the dielectric stack was developed.
Then the elevator-
Falling off the metal layerPECVD (plasma-
Enhanced chemical vapor deposition)-
Silica thick by 2 μm (RIu2009=u20091. 46)
As the bottom cladding, 7 μm thick silicon oxygen nitrogen (RIu2009=u20091. 52)
As the core, another 2 μm thick silica is used as the upper cladding.
Media waveguide is an attractive solution for Integrated Biomedical optics.
Unlike polymers, dielectric materials are resistant to ion and enzyme environments and provide negligible degradation.
Compared with some polymer waveguide (SU-8, PDMS)
They don't absorb the light in ultraviolet rays. blue range.
Since the RI of the wave duct film determines the NA of the wave duct, the PECVD process is carefully optimized to tune the wave duct NA while maintaining the film stress (
72 mpa stretching of silicon oxygen nitrogen and mpa compression of silica)
And uniformity ( 50 μ v; L-ratio